Micronutrients in food have been found to have chemopreventive effects, supporting the conclusions from
epidemiologie studies that consumption of fresh fruits and vegetables reduces cancer risk. The present study
was carried out to evaluate the role of querctin (Q) and sodium gluconate (GNA) supplementation separately
or in combination in ameliorating promotion of colon tumor development by dimethyl-hydrazine (DMH) in
mice. Histopathological observation of colons in mice treated with DMH showed goblet cell dysplasia with
inlammatory cell iniltration. This pathological inding was associated with signiicant alteration in oxidative
stress markers in colon tissues and carcinoembryonic antigen (CEA) levels in plasma. Mice co-treated with GNA
and Q showed mild changes of absorptive and goblet cells and inlammatory cell iniltration in lamina properia,
with improvement in oxidative stress markers. In conclusion, indings of the present study indicate signiicant
roles for reactive oxygen species (ROS) in pathogenesis of DMH-induced colon toxicity and initiation of colon
cancer. Also, they suggest that Q, GNA or the combination of both have a positive beneicial effect against DMH
induced colonic cancer induction in mice.